Journal: Stem Cell Research & Therapy

Article Title: Mouse embryonic palatal mesenchymal cells maintain stemness through the PTEN-Akt-mTOR autophagic pathway

doi: 10.1186/s13287-019-1340-8

Figure Lengend Snippet: The properties of passage 1 MEPM cells. a Immunofluorescence staining of MEPM cells for vimentin (green), nestin (red), HNK-1 (green) (white arrow), and keratin (not stained). b Immunofluorescence staining of MEPM cells for PDGFRα (green), Efnb1 (red), Osr2 (red), and Meox2 (red). c Transwell assays showing that PDGFA (0.5 ng/mL and 5 ng/mL) stimulates chemotaxis of MEPMs. N = 9; * P < 0.05; ** P < 0.01. Cell nuclei (blue) were counterstained with DAPI, and HNK-1 (red) in cytoplasm was stained with Evans blue dye. Scale bar: 20 μm

Article Snippet: Briefly, cells were incubated overnight at 4 °C with mouse mesodermal marker vimentin (1:100; ab92547; Abcam, USA), mouse pan-keratin (1:300; #4545T; Cell Signaling Technology), mouse ectodermal marker nestin IgG1 (1:300; ab11306; Abcam, USA), rabbit autophagosome protein [ ], LC3A/B (1:100; #12741S; Cell Signaling Technology, USA), mouse neural crest marker HNK (1:100; sc81633; Santa Cruz Biotechnology, USA), monoclonal primary antibodies, PDGFRα (1:500; ab203491; Abcam, USA), Ephrin-B1(Efnb1) (1:10; sc515264; Santa Cruz Biotechnology, USA), Osr2 (1:10; sc81971; Santa Cruz Biotechnology, USA), and Meox2 (1:10; sc393516; Santa Cruz Biotechnology, USA) or PBS vehicle alone (negative control).

Techniques: Immunofluorescence, Staining, Chemotaxis Assay

Journal: Stem Cell Research & Therapy

Article Title: Mouse embryonic palatal mesenchymal cells maintain stemness through the PTEN-Akt-mTOR autophagic pathway

doi: 10.1186/s13287-019-1340-8

Figure Lengend Snippet: Immunophenotyping of MEPM cells. a Raw flow cytometry profiles of MEPM cells. b Semi-quantitative profiles from raw flow cytometry data showing cell surface marker expression levels in MEPM cells. Isotype gating using IgG-FITC and IgG-PE controls was performed to minimize non-specific signal

Article Snippet: Briefly, cells were incubated overnight at 4 °C with mouse mesodermal marker vimentin (1:100; ab92547; Abcam, USA), mouse pan-keratin (1:300; #4545T; Cell Signaling Technology), mouse ectodermal marker nestin IgG1 (1:300; ab11306; Abcam, USA), rabbit autophagosome protein [ ], LC3A/B (1:100; #12741S; Cell Signaling Technology, USA), mouse neural crest marker HNK (1:100; sc81633; Santa Cruz Biotechnology, USA), monoclonal primary antibodies, PDGFRα (1:500; ab203491; Abcam, USA), Ephrin-B1(Efnb1) (1:10; sc515264; Santa Cruz Biotechnology, USA), Osr2 (1:10; sc81971; Santa Cruz Biotechnology, USA), and Meox2 (1:10; sc393516; Santa Cruz Biotechnology, USA) or PBS vehicle alone (negative control).

Techniques: Flow Cytometry, Marker, Expressing